ATTEMPTS TO CONTROL PHENOLIC EXUDATION FROM NODAL EXPLANTS OF MANGIFERA INDICA DURING IN VITRO CULTURE

The release of phenolic compounds from many plants causes death of explants within a few days of in vitro culture. This happens due to the toxic effect of phenolics released in media. These phenolics thus need to be controlled for establishing explants inasepticculture. In the present study, phenolic release in the culture media was encountered during nodal culture of Mangifera indica. The exudation of phenolic compounds from nodal explants caused browning within 7-8 days and ultimately they died in 25-30 days of culture. Various strategies were applied to control phenolics released in the media so that cultures could be established. Different experiments using antioxidants, adsorbing agents and liquid media were conducted and the results are presented here.